Effect of intraoperative trypan blue on lens epithelial cells – Histomorphological analysis
نویسندگان
چکیده
منابع مشابه
Crystalline lens capsule staining with trypan blue.
A 1-step method for staining the anterior lens capsule with trypan blue is described. The dye is instilled via a paracentesis port at the start of cataract extraction. As aqueous humor is allowed to exit the anterior chamber (AC), which consequently shallows, the resulting pupil block confines the dye to the AC. An ophthalmic viscosurgical device (OVD) is used to flush dye-stained aqueous from ...
متن کاملEffects of trypan blue on cell viability and gene expression in human retinal pigment epithelial cells.
AIM To evaluate the effects of trypan blue on cell viability and gene expression in human retinal pigment epithelial (RPE) cells. METHODS Three concentrations (0.06 mg/ml, 0.6 mg/ml, and 4 mg/ml) of trypan blue were applied to human ARPE19 cells for 1 minute. Cell viability was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. RPE cells were sampled ...
متن کاملComparative toxicology of trypan blue, brilliant blue G, and their combination together with polyethylene glycol on human pigment epithelial cells.
PURPOSE To determine the toxicity in ARPE-19 human retinal pigment epithelium cells of trypan blue (TB) at 0.15% and 0.25% concentration, brilliant blue G (BBG) at 0.025% and 0.05%, their combination, and the effect of the addition of 4% polyethyleneglycol (PEG), as an additive for increasing the density and thus improving the staining in internal limiting membrane removal, on the individual dy...
متن کاملEvaluation of MTT and Trypan Blue assays for radiation-induced cell viability test in HepG2 cells
Background: Cell viability is an important factor in radiation therapy and thus is a method to quantify the effect of the therapy. Materials and Methods: The viability of human hepatoma (HepG2) cells exposed to radiation was evaluated by both the MTT and Trypan blue assays. The cells were seeded on 96 well-plates at a density of 1 x 104 cells/well, incubated overnight, and irradiated with...
متن کاملthe effect analysis of proficiency on language - switching in iranian efl learners writing
abstract in a protocol analysis of second language writing from 20 adult english as a foreign language (efl) iranian students, this research observed how language-switching (l-s), i.e., first language use in l2 writing, was affected by l2 proficiency. switching interactively between first (l1) and second (l2) languages has been recognized as one of the salient characteristics of l2 writing....
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ژورنال
عنوان ژورنال: Saudi Journal of Ophthalmology
سال: 2019
ISSN: 1319-4534
DOI: 10.1016/j.sjopt.2018.12.006